Green synthesis of silver nanoparticles using Lippia nodiflora aerial extract and evaluation of their antioxidant, antibacterial and cytotoxic effects

Silver nanoparticles biosynthesis has received increasing attention in the field of nanotechnology due to
their antimicrobial and biomedical applications. Green synthesis of metal nanoparticles is anticipated as
a cost effective and eco-friendly alternative in the current research scenario. With this aim, the aqueous
extracts made from the aerial parts of Lippia nodiflora were used as the reducing agents to synthesize
silver nanoparticles (AgNPs) and their antioxidant, antibacterial and cytotoxic properties have also been
evaluated. The biosynthesized AgNPs were characterized by UV–Visible Spectroscopy, Fourier-Transform
Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD) analysis. The AgNPs were found to be stable at
−25.2 mV through zeta potential study. The morphology and size of synthesized silver nanoparticles were
confirmed by Scanning Electron Microscope with energy dispersive spectra (SEM-EDX) and Transmission
Electron Microscopy (TEM) analysis with size range from 30 to 60 nm. Biosynthesized AgNPs exhibited
strong antioxidant activity as well as showed potent antibacterial activity against human pathogenic bacteria.
The cytotoxicity study of AgNPs was also revealed against MCF-7 breast cancer cell lines in a dosedependent
manner. The recognized bioactivity confirmed by the synthesized AgNPs directs towards the
clinical use as an antioxidant, antibacterial and cytotoxic agent.